[Overexpression of tuftelin and KLF-5 and its clinicopathological features in hepatitis B virus-related hepatocellular carcinoma].

Objective: To analyze and evaluate the expressions and clinical value of tuftelin (TUFT1) and Krüppel-like factor 5 (KLF5) in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) tissues. Method: KLF5 mRNA and TUFT1 mRNA transcriptional status in cancer and non-cancer groups were compared according to the Cancer Genome Atlas (TCGA) database. The differences and prognostic value between the groups were analyzed. Postoperative liver cancer and its paired pericancerous tissues, with the approval of the ethics committee, were collected to build tissue chips. The expression of KLF5 and TUFT1 and their intracellular localization were verified by immunohistochemistry. Tissue expression and clinicopathological characteristics were analyzed by immunoblotting. SPSS software was used to analyze the relationship between SPSS and patient prognosis. Results: The transcription level of TUFT1 or KLF5 mRNA was significantly higher in the HCC group than the non-cancer group (P < 0.001), according to TCGA data. Immunohistochemistry and Western blotting examination confirmed the overexpression of TUFT1 and KLF5 in human HCC tissues, which were mainly localized in the cytoplasm and cell membrane. The positivity rates of TUFT1 and KLF5 were 87.1% ( χ(2) = 18.563, P < 0.001) and 95.2% ( χ(2) = 96.435, P < 0.001) in HCC tissues, and both were significantly higher than those in the adjacent group. The expression intensity was higher in stage III-IV than stage I-II of the International Union Against Cancer standard (P < 0.01). The clinicopathological features showed that the abnormalities of the two were significantly related to HBV infection, tumor size, extrahepatic metastasis, TNM stage, and ascites. Univariate analysis was related to tumor size, HBV infection, and survival. Multivariate analysis was an independent prognostic factor for patients with HCC. Conclusion: TUFT1 and KLF5 may both be novel markers possessing clinical value in the diagnosis and prognosis of HBV-related HCC.

Ethnic Disparities in Dental Caries among Adolescents in China.

Comprehensive research on ethnic disparities in dental caries in China is limited. The aims of this cross-sectional study were to compare the levels of dental caries in adolescents between the Han ethnic group and ethnic minority groups in China and to explore the risk indicators for dental caries within ethnic subgroups. Data from the Fourth National Oral Health Survey in 2015, which covered all 31 province-level administrative divisions in mainland China, were used. The dental caries status in the permanent dentition of adolescents aged 12, 13, 14, and 15 y was measured using the decayed, missing, and filled teeth (DFMT) score, and sociodemographic characteristics and oral health-related behaviors were also collected. A total of 118,601 adolescents were included, with ethnic minority groups accounting for 13.15%. Of the Han and minority groups, the standardized prevalence of dental caries experience was 40.58% and 47.67%, and the mean DMFT scores were 0.97 and 1.28, respectively. According to the multivariate zero-inflated negative binomial regression analysis, the caries status of minorities was more severe than Han adolescents (adjusted prevalence rate ratio [PRR], 1.14; 95% confidence interval [CI], 1.10-1.18). This disparity was greater among adolescents who lived in rural areas, had mid-level economic status, and frequently consumed sugary beverages. After propensity score matchings, Uygur (PRR, 1.44; 95% CI, 1.25-1.67), Tibetan (PRR, 1.39; 95% CI, 1.3-1.48), and Yi (PRR, 1.24; 95% CI, 1.04-1.48) adolescents were significantly more likely to have caries than Han adolescents. Subgroup analyses revealed that gender, age, location of residence, economic status, region, consumption of sweet snacks and sugary beverages, and dental visit pattern were significantly associated with dental caries within ethnic minorities.

[Contemporary dietary patterns and oral disease].

The achievements and breakthroughs in scientific field have provided scientific evidence for the relationship amongst diet, nutrition and chronic diseases, including oral diseases. Thus many international organizations and the governments of most countries were pushed to pay their attention to the prevention of chronic diseases by dietary patterns. From 1989 to 2018, the Food and Agriculture Organization of the United Nations and World Health Organization organized expert consultations for many times to issue a series of global strategies and action plans on diet and health. The governments of China, Japan and US had produced the Dietary Guidelines for Residents. The results showed that the morbidity and mortality of many chronic diseases had been decreased by following the dietary guidance. However, the scientific achievements did not seem to produce tremendous enhancement in public health for various reasons. In the present article, the authors analyses the relationship between contemporary dietary patterns and oral health, and make suggestions on preventing and controlling oral diseases via dietary patterns for policy makers and dental professionals.

[Quantitative detection and clinical value of CD44 in patients with nonalcoholic fatty liver disease].

Objective: To quantitatively detect CD44 expression in patients with nonalcoholic fatty liver disease (NAFLD) for comparative analysis. Methods: Patients with chronic liver diseases accompanied with or without NAFLD, including chronic hepatitis B, cirrhosis and hepatocellular carcinoma after chronic hepatitis B, and healthy blood donors as normal controls who admitted to the Affiliated Hospital of Nantong University from May to October 2018 were selected. The proportion of CD44 positive cells was analyzed by flow cytometry. CD44 level was quantified by an enzyme-linked immunosorbent assay, and the biochemical indicators such as serum aspartate aminotransferase, alanine aminotransferase activity, total cholesterol and triglyceride were routinely analyzed. The cancerous and adjacent cancerous tissues of patients accompanied with or without NAFLD were collected by self-matching method and analyzed by immunoblotting and histochemistry and compared by CD44 integrated optical density. Image-Pro Plus version 6.0, Image J, GraphPad Prism 5.0, Photoshop, Microsoft Excel and IBM SPSS statistics 23 were used to analyze and draw pictures. An independent sample t-test was used to compare the differences between groups. Results: Patients accompanied with NAFLD had hepatocyte injury and dyslipidemia. NAFLD and chronic liver disease patients had significantly elevated serum CD44 levels than normal control group (P < 0.01). CD44 positive lymphocyte ratio was 78.19 % ± 16.33 % in NAFLD patients and 68.47% ± 20.91% in chronic hepatitis B group, which was higher than the control group (46.51% ± 20.52%). Chronic hepatitis B group with steatosis had significantly higher CD44 concentration (181.42 ± 49.36) ng/ml than chronic hepatitis B group (142.52 ± 53.87) ng/ml and normal control group (99.47 ± 15.23) ng/ml. CD44/GAPDH ratio in the liver cancer group (1.306 ± 0.614) was significantly higher than paracancerous group (0.477 ± 0.291) and the difference between the two groups was statistically significant (t = 3.451, P = 0.004). The integrated optical density of CD44 in the NAFLD-related liver cancer and paracancerous group were 25.721 ± 5.881 and 14.155 ± 4.001 and the difference between the groups was statistically significant (t = 14.544, P < 0.001). The pathological features of high expression of CD44 in patients with hepatocellular carcinoma were significantly correlated with HBV infection, tumor size, single/multi-center, and lymph node metastasis, degree of differentiation, TNM grade, Child-Pugh score, portal vein tumor thrombus and extrahepatic metastasis. HCC patients with NAFLD had significantly higher serum CD44 (234.62 ± 69.40) ng/ml than patients without NAFLD (186.49 ± 58.89) ng/ml (t = -3.191, P = 0.002), but there was no statistically significant difference in the clinicopathological characteristics between the high/low CD44 groups of HCC patients with NAFLD. Conclusion: The results suggest that CD44 is abnormally activated and its mechanism may play an important role in the progression of NAFLD.

The prevalence of and risk factors for non-carious cervical lesions in adults in Hubei Province, China.

OBJECTIVES: To describe the prevalence of non-carious cervical lesions (NCCLs) and to assess the relative affects of risk factors on NCCLs in middle-aged and elderly people in Hubei Province, China. DESIGN: A sample of 2,160 adults, aged 35-44 years and 65-74 years and balanced by age, gender, and urbanization, participated in the cross sectional epidemiological survey. Non-carious cervical lesions were examined using a modified Tooth Wear Index. Data were collected based on structured questionnaires that assessed general information as well as oral health. RESULTS: The prevalence of non-carious cervical lesions was 38.8% for 35-44-year-olds and 56.6% for 65-74-year-olds. The first premolars, canines, and second premolars showed the highest prevalence of lesions, while the second molars demonstrated the least. Several risk factors such as age (OR = 2.45, p < 0.001), location (OR = 1.68, p = 0.001), frequency of toothbrushing (OR = 1.33, p = 0.016), bruxism (OR = 1.37, p < 0.001), and family income (OR = 1.44, p < 0.001) were found to be associated with lesion occurrence. CONCLUSIONS: The prevalence of non-carious cervical lesions was relatively high in the middle-aged and elderly persons in China and was also associated with socio-behavioural risk factors.

A two-year randomized clinical trial of chlorhexidine varnish on dental caries in Chinese preschool children.

The objective of this study was to assess the effect of six-monthly professional applications of chlorhexidine varnish on the prevention of dental caries in primary molars in Chinese preschool children. In a double-blinded, randomized, placebo-controlled clinical trial, 334 children aged 4-5 years were randomly divided into two groups. Children in the test group received six-monthly applications of a 40% chlorhexidine varnish, and the control children received a placebo varnish. Caries status of the children was assessed by two calibrated examiners at baseline and after 24 months, according to criteria recommended by the World Health Organization. The two-year mean caries increments in the test and the control group children were 1.0 and 1.6 decayed, missing, or filled molar surfaces (dmfs-molar), respectively, a 37.3% reduction (t test, p = 0.036). No side-effects were found. It was concluded that six-monthly applications of chlorhexidine varnish were effective in reducing the incidence of dental caries in primary molars.

The effect of a bi-annual professional application of APF foam on dental caries increment in primary teeth: 24-month clinical trial.

The purpose of this study was to evaluate the effect of a bi-annual professional application of acidulated phosphate fluoride (APF) foam on caries increment in the primary dentition over a two-year period in the People's Republic of China. In a double-blind, cluster-randomized, placebo-controlled trial, 392 children aged 3-4 years from 15 classes were randomly assigned to two groups on a school class basis. The experimental group (8 classes) received a bi-annual APF foam application, and the control group (7 classes) received the placebo. The mean increment of dmfs in the experimental group was 24.2% lower than that in the control group (p < 0.05). The significant caries reduction was observed on approximal surfaces in the experimental group compared with the control group (p < 0.01), but there were no differences on occlusal surfaces (p > 0.05). A bi-annual professional application of APF foam was effective in reducing the increment of dental caries in the primary teeth.

Changes in oral health knowledge and behaviour 1987-95 among inhabitants of Wuhan City, PR China.

The purpose of this study was to evaluate the changes of oral health knowledge and behaviour among Wuhan citizens after six years of 'Love Teeth Day' campaigns. Representative samples of citizens aged 10-62 years were identified and interviewed in 1987 (n = 1180) and in 1995 (n = 1014). Data on oral health knowledge and practises were collected by structured questionnaires in both surveys and high response rates were obtained (87-94 per cent). In general, improvements in oral health knowledge and toothbrushing behaviour were observed in 1995 as compared with 1987. The younger age group (10-19-year-olds) showed significantly higher improvements of positive answers to knowledge items and toothbrushing behaviour. The findings also indicated that less than half of the interviewees were aware of the anti-caries effect of fluoride and about 30 per cent of the participants brushed their teeth performing the recommended method (vertical). In China, community based oral health education programmes should be implemented to further improve regular self-care practises, especially in relation to the adult population.

Oral health status and oral health behaviour of 12-year-old urban schoolchildren in the People's Republic of China.

OBJECTIVE: To describe oral health status of urban Chinese children aged 12 years, to evaluate the pattern of oral health behaviour, and to assess the relative effect of socio-behavioural risk factors on dental caries experience. DESIGN: A cross-sectional survey of 12-year-old children was conducted in the Hubei Province of China, including urban and periurban groups. The sampling was in accordance with the WHO procedures as applied in the second national survey of oral health. Data were collected by clinical examinations and use of self-administered questionnaires. SETTING: The survey was initiated by the National Committee of Oral Health, China, in order to aid the planning and evaluation of school-based oral health care. SUBJECTS: A sample of 698 children aged 12 years (96 per cent of original sample) participated in the survey; the sample was balanced by gender and urbanisation (urban/periurban). OUTCOME MEASURES: The children were clinically examined according to the WHO basic methods and responded to standardised questionnaires on oral health behaviour, knowledge, and attitudes. RESULTS: The mean DMFT of the children was 0.77 and DT constituted most of the caries index. About 65 per cent of the children had CPITN maximum score 2 (gingival bleeding and calculus). Only 40 per cent brushed their teeth at least twice a day and 46 per cent had seen a dentist within the past year. Variations by urbanisation were observed and the multiple linear regression of dental caries experience revealed that consumption of surgary foods, location and dental visits were the most important independent variables. CONCLUSION: This study has shown that systematic oral health promotion programmes for children in China are urgently needed.